Application of a Nested Polymerase Chain Reaction Assay to Detect Mycoplasma Hyopneumoniae from Nasal Swabs
نویسندگان
چکیده
منابع مشابه
A novel polymerase chain reaction assay to detect Mycoplasma genitalium.
AIMS To design and validate a polymerase chain reaction (PCR) assay targeting the 16S rRNA gene of Mycoplasma genitalium. METHODS Primers were designed that were complementary to the 16S rRNA gene sequence of M genitalium. After optimisation of the reaction conditions, the PCR was tested against nine M genitalium strains, a dilution series of M genitalium DNA, and a panel of common microorgan...
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Fifty-four samples were collected from growing and finishing pigs for the molecular diagnosis of enzootic porcine pneumonia. Nineteen lung fragments were obtained from pigs that showed signs of respiratory disease and 35 nasal swabs were obtained from clinically healthy pigs. For the detection of the bacterial genome in the samples, the nested PCR technique was used to amplify a fragment of 706...
متن کاملDetection of Mycoplasma hyopneumoniae by ELISA and nested PCR from blood samples and nasal swabs from pigs in Slovakia
The aim of our study was to map the situation of swine mycoplasmoses on four farms in the region of Eastern Slovakia. The primary agent of Enzootic pneumonia of swine is Mycoplasma hyopneumoniae. After reviewing the health status of conventional herds and evaluation of clinical symptoms, paired samples of nasal swabs and venous blood samples were collected from 38 pigs with clinical signs of re...
متن کاملDetection of Mycoplasma hyopneumoniae by polymerase chain reaction in swine presenting respiratory problems
Since Mycoplasma hyopneumoniae isolation in appropriate media is a difficult task and impractical for daily routine diagnostics, Nested-PCR (N-PCR) techniques are currently used to improve the direct diagnostic sensitivity of Swine Enzootic Pneumonia. In a first experiment, this paper describes a N-PCR technique optimization based on three variables: different sampling sites, sample transport m...
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Canine parvovirus 2 (CPV-2) is the causative agent of acute hemorrhagic enteritis and myocarditis in dogs. A nested polymerase chain reaction (n-PCR) was developed using published pCPV-2ab as external primer set and self designed and custom synthesized pCPV-2N as internal primer set for the detection of canine parvovirus in faecal samples of dogs. Out of 52 faecal samples, 27 and 31 were tested...
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ژورنال
عنوان ژورنال: Journal of Veterinary Diagnostic Investigation
سال: 1999
ISSN: 1040-6387,1943-4936
DOI: 10.1177/104063879901100307